3D Cell Culture - Bridging the gap in discovery
Decades ago, when human cells were successfully cultured and grown outside the human body in a lab environment, it paved the way for the screening of drugs as potential anti-cancer drugs. A number of drugs reached the market for the benefit of the patients through such experimentations on cell lines. But soon a major drawback was noted by researchers; many promising results obtained in cell culture experiments were not translated to its desired effectiveness in animal experiments and human trials. The major reason for this observation was that cells in our body are in a three dimensional environment, while the cells in the lab were growing on flat culture dish surfaces. The spatial arrangement of cells in the body determines how they interact and communicate with each other and their microenvironment.
Three important characteristics of ligo cells scaffolds are its ease of use, reliability and versatility. The ligocells scaffolds can be used in any normal cell culture lab where cell lines are being grown and no specialized equipment are needed for using ligocells scaffolds. An easy to follow step wise interpretation given in the link to LigoCells website. Five steps to 3D cell culture with ligocells, will ensure consistent and reliable growth of cells on the scaffolds. Once the cells are grown on the scaffolds, they are amenable to a wide variety of experimental tests and assays. Ligocells being made of non-protein based biodegradable polymer ensures minimal interference from the material in the experimental results. It is also well suited to be subjected to histopathology techniques and staining. The ligocells scaffolds can be used in either the traditional static cell culture mode or in a dynamic environment of a bioreactor.