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3D cell culture scaffold

ligo cells are biodegradable and biocompatible polylactic acid based scaffolds for 3D cell culture


3D Cell Culture - Bridging the gap in discovery

Decades ago, when human cells were successfully cultured and grown outside the human body in a lab environment, it paved the way for the screening of drugs as potential anti-cancer drugs. A number of drugs reached the market for the benefit of the patients through such experimentations on cell lines. But soon a major drawback was noted by researchers; many promising results obtained in cell culture experiments were not translated to its desired effectiveness in animal experiments and human trials. The major reason for this observation was that cells in our body are in a three dimensional environment, while the cells in the lab were growing on flat culture dish surfaces. The spatial arrangement of cells in the body determines how they interact and communicate with each other and their microenvironment.

Three important characteristics of ligo cells scaffolds are its ease of use, reliability and versatility. The ligocells scaffolds can be used in any normal cell culture lab where cell lines are being grown and no specialized equipment are needed for using ligocells scaffolds. An easy to follow step wise interpretation given in the link to website. Five steps to 3D cell culture with ligocells, will ensure consistent and reliable growth of cells on the scaffolds. Once the cells are grown on the scaffolds, they are amenable to a wide variety of experimental tests and assays. Ligocells being made of non-protein based biodegradable polymer ensures minimal interference from the material in the experimental results. It is also well suited to be subjected to histopathology techniques and staining. The ligocells scaffolds can be used in either the traditional static cell culture mode or in a dynamic environment of a bioreactor.


In turn, these signals affect differentiation, proliferation and range of other cellular functions. This led various scientists to try growing cells on scaffolds and matrices to obtain 3D cell cultures and the results obtained were seen to be more reliable 1. Thus, there is a shift happening among scientists, especially among cancer researchers, to grow cells on scaffolds to obtain 3D cell cultures to be used for drug screening and other experiments. This demand led to the launch of variety 3D cell culture scaffolds by biotech companies. One thing that deters researchers from using 3D cell cultures routinely is its labour intensive protocols. We have made the growth of cells on LigoCellsscaffold in an easy to follow format which can be followed in a standard cell culture lab. Use of 3D cell cultures can easily spot promising compounds for cancer in the early stages of experimentation itself and weed out the less promising ones. LigoCells scaffolds, being made of the biodegradable and biocompatible polymer, Polylactic acid (PLA), can be directly implanted in animals to carry out in vivo experiments. This seamless integration of in vitro and in vivo experiments can result in faster, better and cost effective discovery process!

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The science of

are biodegradable and biocompatible polylactic acid based scaffolds for 3D cell culture.

LigoCells scaffolds are made up of a biodegradable and biocompatible polymer, Polylactic acid (PLA). PLA and its related polymers is one of the most widely used polymers by the scientific community for fabricating scaffolds and matrices to grown cells for tissue engineering purposes. Thus, PLA continues to be one of the most accepted materials of choice for making scaffolds for growth of cells in a three dimensional environment. It degrades in the in vitro and in vivo environment into lactic acid and subsequently into carbon dioxide and water, which are harmless to the cells. LigoCells scaffolds are made of PLA of the highest quality and there are no other leachable chemicals and excipients in the scaffolds to harm the cells.

Two important aspects of scaffold design are its surface properties and its topology; both at its micro scale and at its larger dimensions. These parameters should suit and promote cellular attachment and growth of cells into three dimensional cultures. Cells have evolved over millions of years adapted to very stringent and specific conditions that will enable its growth, organization and communication in a three dimensional environment.

The unique architecture of LigoCells scaffolds consists of porous polymer particles of 150 to 250 micro meter diameter assembled into disc shaped scaffolds suited for culture in a 24 well format. The scaffold floats at the air-media interface and the fluid filled scaffolds ensures a steady supply of media to the growing cells. LigoCells scaffold support the growth of a wide variety of adherent cells and it can be coated with a wide variety materials for specific cell growth like collagen, fibronectin and Matrigel(TM) as the need may arise. After the initial seeding of the scaffolds with the cells optimal three dimensional growth is obtained in 7 to 10 days and this can be used for a variety of experimental tests. The cell-scaffold construct can be stably handed by a sterile forceps and can also be transferred to the dynamic environment of a cell culture bioreactor if required. The LigoCells scaffold can also be implanted in animals to correlate the test results obtained in in vitro to the in vivo situation. Thus, LigoCells scaffold help you connect the in vitro to the in vivo for better discovery!

ABOUT US

Polyskin was found in 2008 by Dr. Rajmohan G. Our primary interest lies in translation of innovations from the Research lab through the various stages of pre-clinical and clinical evaluations, with focus on clinical applications in regenerative medicine

ADDRESS

  • Address: Bio360, LifeScience Park
    Trivandrum, India, 695731
  • Email: info@polyskin.in
  • Website: www.polyskin.in
  • Phone: +91 860 620 9399